ab100004 ACTB antibody
品牌 |
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产品货号 |
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来源种属 |
Rabbit |
抗体克隆 |
Polyclonal |
来源亚型 |
IgG |
实验方法 |
WB,IHC,ELISA |
实验种属 |
Human,Mouse,Rat |
偶联标记 |
Unconjugated |
目的蛋白 |
ACTB |
产品规格 |
25μl,100μl,200μl |
产品报价 |
¥700/¥1600/¥2600 |
实验应用
Immunohistochemistry
Predicted cell location: Cytoplasm Positive control: Human tonsil
Recommended dilution: 50-100
Western blotting
Predicted band size: 42 kDa
Positive control: Mouse kidney tissue, Mouse brain tissue, 231 cell, Hela cell, 293T cell, HepG2 cell, NIH/3T3 cell
lysates
Recommended dilution: 1000-5000
ELISA
Recommended dilution: 5000-10000
最佳稀释倍数与浓度应由实验研究人员确认
产品说明
产品背景
产品背景
This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, integrity, and intercellular signaling. The encoded protein is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins that are ubiquitously expressed. Mutations in this gene cause Baraitser-Winter syndrome 1, which is characterized by intellectual disability with a distinctive facial appearance in human patients. Numerous pseudogenes of this gene have been identified throughout the human genome. (The background information is obtained from official databases and does not represent the product information.)
Name of antibody:
ACTB
Immunogen:
Synthetic peptide of human ACTB
Full name:
actin beta
Synonyms:
BRWS1; PS1TP5BP1
SwissProt:
P60709
ELISA Recommended dilution:
5000-10000
IHC positive control:
Human tonsil
IHC Recommend dilution:
50-100
WB Predicted band size:
42 kDa
WB Positive control:
Mouse kidney tissue, Mouse brain tissue, 231 cell, Hela cell, 293T cell, HepG2 cell, NIH/3T3 cell lysates
WB Recommended dilution:
1000-5000
产品图片
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The image on the left is immunohistochemistry of paraffin-embedded Human tonsil tissue using ab100004(ACTB Antibody) at dilution 1/50, on the right is treated with synthetic peptide. (Original magnification: ×200) |
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The image on the left is immunohistochemistry of paraffin-embedded Human tonsil tissue using ab100004(ACTB Antibody) at dilution 1/50, on the right is treated with synthetic peptide. (Original magnification: ×200) |
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Gel: 8%SDS-PAGE, Lysate: 40 μg, Lane 1-7: Mouse kidney tissue, Mouse brain tissue, 231 cell, Hela cell, 293T cell, HepG2 cell, NIH/3T3 cell lysates, Primary antibody: D110001(ACTB Antibody) at dilution 1/800, Secondary antibody: ab100004(HRP-conjugated Goat anti rabbit IgG) at 1/5000 dilution, Exposure time: 5 seconds |
