ab100002 GAPDH antibody
品牌 |
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产品货号 |
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来源种属 |
Mouse |
抗体克隆 |
Monoclonal |
来源亚型 |
IgG1 |
实验方法 |
WB,IF |
实验种属 |
Human,Mouse,Rat |
偶联标记 |
Unconjugated |
目的蛋白 |
GAPDH |
产品规格 |
25μl,100μl,200μl |
产品报价 |
¥700/¥1600/¥2600 |
实验应用
Immunohistochemistry
Predicted cell location: Cytoplasm
Positive control: Human colorectal cancer and Human
ovarian cancer
Recommended dilution: 100-500
Western blotting
Predicted band size:36 kDa
Positive control:HUVEC cell and Human left lung tissue lysates
Recommended dilution: 1000-5000
免疫印迹（WB），免疫组织化学（IHC）
最佳稀释倍数与浓度应由实验研究人员确认
最佳稀释倍数与浓度应由实验研究人员确认
产品说明
产品背景
产品背景
This gene encodes a member of the glyceraldehyde-3-phosphate dehydrogenase protein family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The encoded protein has additionally been identified to have uracil DNA glycosylase activity in the nucleus. Also, this protein contains a peptide that has antimicrobial activity against E. coli, P. aeruginosa, and C. albicans. Studies of a similar protein in mouse have assigned a variety of additional functions including nitrosylation of nuclear proteins, the regulation of mRNA stability, and acting as a transferrin receptor on the cell surface of macrophage. Many pseudogenes similar to this locus are present in the human genome. Alternative splicing results in multiple transcript variants.
保存缓冲液
Ascitic fluid with 0.05% sodium azide and 40% glycerol
运输条件
2-8°C
靶点 Uniprot 登记号
P04406
靶点基因 ID
2597
靶点全称
glyceraldehyde-3-phosphate dehydrogenase
靶点别名
G3PD; GAPD; HEL-S-162eP
靶点研究领域
Metabolism, Cancer, Neuroscience
亚型
IgG1
产品图片
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The image is immunohistochemistry of paraffin-embedded Human colorectal cancer and Human ovarian cancer tissue using ab100002(GAPDH Antibody) at dilution 1/150. (Original magnification: ×200) |
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Gel: 8%SDS-PAGE, Lysate: 40 μg, Lane 1-2: HUVEC cell and Human left lung tissue lysates, Primary antibody: ab100002(GAPDH Antibody) at dilution 1/1200, Secondary antibody: Goat anti mouse IgG at 1/5000 dilution, Exposure time: 3 minutes |
