ab100001 6×His Tag antibody
品牌 |
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产品货号 |
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来源种属 |
Mouse |
抗体克隆 |
Monoclonal |
来源亚型 |
IgG1 |
实验方法 |
WB,IF,IP |
实验种属 |
Human,Mouse,Rat,Rabbit,Cow,Pig,Dog |
偶联标记 |
Unconjugated |
目的蛋白 |
6×His Tag |
产品规格 |
25μl,100μl,200μl |
产品报价 |
¥700/¥1600/¥2600 |
实验应用
Western blotting
Predicted band size: 50 kDa
Positive control: Fusion proteins containing the His Tag
Recommended dilution: 1000-5000
Immunofluorescence
IF Positive control: 3T3 cells
Recommended dilution: 100-500
Immunoprecipitation
Recommended dilution: 100-200
免疫印迹（WB），免疫荧光（IF），免疫沉淀（IP）
最佳稀释倍数与浓度应由实验研究人员确认
最佳稀释倍数与浓度应由实验研究人员确认
产品说明
产品背景
产品背景
The 6 X histidine motif is used as a Tag on many recombinant proteins to facilitate purification. It can then be purified using immobilized metal ions, traditionally nickel, in a procedure termed immobilized-metal affinity chromatography. Further analysis of the protein can be achieved using an antibody specific to the 6 X histidine in techniques such as western blotting. The antibody recognizes the His-Tag fused to targeted proteins in transfected or transformed cells.
保存缓冲液
Ascitic fluid with 0.05% sodium azide and 40% glycerol
运输条件
2-8°C
靶点全称
HHHHHH Tag
靶点别名
6 His epitope Tag
靶点研究领域
Cell Biology
亚型
IgG1
产品图片
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Gel: 8%SDS-PAGE, Lysate: 2 μg, Lane: Fusion proteins containing the His Tag, Primary antibody: ab100001(6×His Tag Antibody) at dilution 1/3000, Secondary antibody: Goat anti mouse IgG at 1/5000 dilution, Exposure time: 1 minute |
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The image is immunofluorescence of methanol-fixed 3T3 cells transfected with His-TAF4B vector using ab100001(6×His Tag Antibody) at dilution 1/500 |
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Immunoprecipitation analysis: Lane 1 is Transfected cell lysate(Input), Lane 2 is Transfected cell lysate using ab100001(6×His Tag Antibody) at dilution 1/200, Lane 3 is Normal Mouse IgG(Control) |
