SAB10005 Goat anti-Human IgG (H+L),HRP

WB 1:1000

ELISA 1:250

IHC  1:50 
 

WB, Western blot; ELISA, Enzyme-linked Immunosorbent Assay; IHC, Immunohistochemistry.

保存条件：

-20℃保存，一年有效。4℃保存，半年有效。

注意事项：

1、叠氮钠会抑制辣根过氧化物酶的活性，在含有辣根过氧化物酶的二抗中不可加入叠氮钠。

2、本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。

3、为了您的安全和健康，请穿实验服并戴一次性手套操作。

最佳稀释倍数与浓度应由实验研究人员确认

产品背景

Antibodies, also known as immunoglobulins (Igs) are critical for immunity and are grouped into five primary classes: IgG, IgM, IgA, IgD, and IgE. The most abundant antibody isotype is immunoglobulin G (IgG) with concentrations ranging from 7.5-22 mg/ml in human serum and has a molecular weight of 150 kDa. The major effector functions of IgG include neutralization, opsonization, complement fixation and antibody dependent cell-mediated cytotoxicity (ADCC). This monomeric immunoglobulin, expressed on the surface of mature B cells, is often depicted as a Y-shape and comprised of 2 heavy chains and 2 light chains linked by disulfide bonds. The heavy chain is type gamma including subtypes gamma 1, gamma 2, gamma 3, and gamma 4 while the light chain is either a kappa or lambda chain. An IgG molecule has two antigen binding sites, each consisting of a heavy and light chain N-terminal variable domain. When combined with the constant heavy chain 1 (Ch1) and the constant light chain domains, it forms the fragment antigen-binding (Fab) region (2 per antibody). The remaining domains (Ch2-Ch4) of both heavy chains make up the Fc region and contain a site for covalently linking an enzymatic or fluorochrome probe, such as HRP or Janelia Fluor 549, for target detection and visualization.

The 4 IgG subclasses, sharing 95% amino acid identity, include IgG1, IgG2, IgG3, and IgG4 for humans and IgG1, IgG2a, IgG2b, and IgG3 for mice. The relative abundance of each human subclass is 60% for IgG1, 32% for IgG2, 4% for IgG3, and 4% for IgG4. In an IgG deficiency, there may be a shortage of one or more subclasses.

本辣根过氧化物酶标记山羊抗人IgG (H+L) (HRP-labeled Goat Anti-Human IgG (H+L))为进口分装，用于Western、ELISA和IHC等的二抗。HRP，即horseradish peroxidase，中文名为辣根过氧化物酶，可以在Western检测时催化ECL类试剂(如 ECL、BeyoECL等)产生化学发光，可以在ELISA时催化TMB产生蓝色，也可以在IHC或Western blot检测时催化DAB产生 棕色沉淀。
 

本辣根过氧化物酶标记山羊抗人IgG (H+L)用于WB、ELISA和IHC的推荐稀释比例参考上表，实际实验操作过程中需根据 抗原和抗体的具体情况适当调节二抗的稀释比例。
对于WB，推荐的稀释范围为1:500-2000。
 

本抗体为用纯化的人IgG免疫山羊，然后用亲和纯化柱对获得的抗血清进行纯化而获得的优质二抗。
 

本抗体如果用于常规的Western检测，以每次检测需10毫升1:1000稀释的二抗计，至少可以检测100次。