SAB10001 Donkey Anti-Goat IgG(H+L),HRP
品牌 |
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产品货号 |
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来源种属 |
Donkey |
抗体克隆 |
Polyclonal |
来源亚型 |
IgG |
实验方法 |
WB,IHC,ELISA |
实验种属 |
Goat |
偶联标记 |
HRP |
目的蛋白 |
Goat IgG(H+L) |
产品规格 |
200μl |
产品报价 |
¥350 |
实验应用
| 来源 | 用途 | WB | ELISA | IHC |
| Donkey | WB, ELISA, IHC | 1:1000-2000 | 1:250-500 | 1:50-100 |
WB, Western blot; IHC, Immunohistochemistry.
保存条件： -20ºC保存，一年有效。4ºC保存，半年有效。
注意事项：
1、叠氮钠会抑制辣根过氧化物酶的活性，在含有辣根过氧化物酶的二抗中不可加入叠氮钠。
2、本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。
3、为了您的安全和健康，请穿实验服并戴一次性手套操作。
使用说明：
Western、ELISA或IHC请参考相关实验步骤进行。起始稀释浓度按照产品简介中推荐的稀释比例进行稀释。
最佳稀释倍数与浓度应由实验研究人员确认
产品说明
产品背景
Antibodies, also known as immunoglobulins (Igs) are critical for immunity and are grouped into five primary classes: IgG, IgM, IgA, IgD, and IgE. The most abundant antibody isotype is immunoglobulin G (IgG) with concentrations ranging from 7.5-22 mg/ml in human serum and has a molecular weight of 150 kDa. The major effector functions of IgG include neutralization, opsonization, complement fixation and antibody dependent cell-mediated cytotoxicity (ADCC). This monomeric immunoglobulin, expressed on the surface of mature B cells, is often depicted as a Y-shape and comprised of 2 heavy chains and 2 light chains linked by disulfide bonds. The heavy chain is type gamma including subtypes gamma 1, gamma 2, gamma 3, and gamma 4 while the light chain is either a kappa or lambda chain. An IgG molecule has two antigen binding sites, each consisting of a heavy and light chain N-terminal variable domain. When combined with the constant heavy chain 1 (Ch1) and the constant light chain domains, it forms the fragment antigen-binding (Fab) region (2 per antibody). The remaining domains (Ch2-Ch4) of both heavy chains make up the Fc region and contain a site for covalently linking an enzymatic or fluorochrome probe, such as HRP or Janelia Fluor 549, for target detection and visualization (1,2,3).
本辣根过氧化物酶标记驴抗山羊IgG (H+L) (HRP-labeled Donkey Anti-Goat IgG (H+L))，用于Western、ELISA和 IHC等的二抗。HRP，即horseradish peroxidase，中文名为辣根过氧化物酶，可以在Western检测时催化ECL类试剂(如ECL、 BeyoECL等)产生化学发光，可以在ELISA时催化TMB产生蓝色，也可以在IHC或Western blot检测时催化DAB产生棕色沉淀。
本辣根过氧化物酶标记驴抗山羊IgG (H+L)用于WB、ELISA和IHC的推荐稀释比例参考上表，实际实验操作过程中需根据抗原和抗体的具体情况适当调节二抗的稀释比例。
例如对于WB，推荐的可以调节的稀释范围为1:500-5000。
本抗体为用纯化的山羊IgG免疫驴，然后用亲和纯化柱对获得的抗血清进行纯化，并经过人、兔、小鼠、大鼠、豚鼠、仓鼠、马、鸡血清吸附纯化的优质二抗。对人、兔、小鼠、大鼠、豚鼠、仓鼠、马、鸡血清蛋白几乎没有结合能力(Minimal Cross-reaction to Human, Rabbit, Mice, Rat, Guinea Pig, Syrian Hamster, Horse and Chicken Serum Proteins)。
本抗体如果用于常规的Western检测，以每次检测需10毫升1:1000稀释的二抗计，可以检测20次。
产品图片
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Western Blot: Donkey anti-Goat IgG (H+L),HRP(SAB10001) - Analysis using Donkey anti-Goat IgG (H+L),HRP using Embryo. |
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Immunohistochemistry-Paraffin: Donkey anti-Goat IgG (H+L),HRP(SAB10001） - Detection of human Sp1 by immunohistochemistry. Sample: FFPE section of human stomach carcinoma. Antibody: Affinity purified rabbit anti-Sp1 used at a dilution of 1:5,000 (0.2ug/ml). Secondary Antibody: Donkey anti-Goat IgG (H+L),HRP(SAB10001) lot 23 (left) and lot 24 (right) used at a dilution of 1:200 (5ug/ml). Detection: DAB |
